Integrated Evaluation and the Physiological and Biochemical Responses of Semi-Wild Cotton under Complex Salt-Alkali Stress

[Objective] The genetic diversity of semi-wild cotton was abundant, so more new elite resistant genes could be found and applied to improve the resistance of cotton. The aim of this study was to primarily study on the regulation of response to salt-alkali stress, explore the method of integrated evaluation and characterize the resistance of semi-wild cotton. [Method] Four semi-wild and two cultivated Gossypium hirsutum accessions were evaluated for phonotypical, physiological and biochemical traits under alkali-salt versus normal conditions in hydroponic solutions. The alkali-salt tolerance was determined based on principal component analysis, cluster analysis, correlation analysis, grey rational analysis and analysis of variance. [Result] On the basis of the overall results, the alkali-salt stress tolerance of the six accessions was ranked as: Marie-galante85 > Latifolium32 > CRI16 > CRI12 > Latifolium40 > Latifolium130. Roots were found to be the most important responsive systems to complex alkali-salt stress. At seedling growth stage, the active scavenging system played a crucial role in response to alkali-salt stress. In addition, the salt tolerant and salt sensitive accessions showed different response tends towards leaf peroxidase, root ascorbate peroxidase and root catalase within 48 h, suggesting the accessions have different levels of response to salt stress. [Conclusion]Our study identified the most alkali-salt tolerant accessions and provided basic concept of complex alkali-salt tolerance mechanism within cotton accessions. And, our study provided a simple and rapid, highly accurate and precise method for evaluating salt resistance of cotton, and proved that the balance of ROS system play an important role in response to salt-alkali stress. Hence, mining of salt tolerant genes from these accessions may facilitate the development of novel salt tolerant variety.     

Conservation needs for the endangered New Zealand sea lion,Phocarctos hookeri

1. Understanding population size and trend is critical information in species management and conservation. To enable accurate population trend estimates, consistent robust monitoring of a species is essential, particularly for a species such as the New Zealand (NZ) sea lion, Phocarctos hookeri , which has experienced an almost continuous decline in pup production since the late 1990s.
2. This research examines the pup production estimates for all known breeding sites for this species, and using a stage‐structured matrix population model, estimates population size and trend between 1995 and 2015.
3. Overall, it is estimated that 2,316 pups were born in 2015, a decrease of 13% since 1995 and a 27% decline since the highest pup production estimate in 1998. This decline has been driven by the significant decline of 48% at the main breeding area, the Auckland Islands since 1998.
4. Using the stage‐structured matrix population model a total species population size of 11,767 sea lions (95% CrI: 10,790–12,923) was estimated. This is the lowest population size of any sea lion species. Trend data for the Auckland Islands indicated that pup and population numbers have decreased at 1.9% yr^?1 in the last 20 yr, while total species population decline is 0.6% yr^?1.
5. Estimates of population trends for this species have been hindered by inconsistent monitoring at most breeding sites. This study strengthens the growing field of research highlighting the need for consistent long‐term monitoring for the conservation management of endangered species.

     

花前氮亏缺对水稻叶片氮代谢酶活性的影响

以籼型杂交稻中浙优1号和籼粳型杂交稻甬优12为材料,试验分析了花前不同时期氮亏缺处理对水稻叶片氮代谢酶活性的影响。结果表明,花前氮亏缺导致植株上3叶氮浓度、NR、GS、GOGAT、GOT和GPT酶活性大幅下降,GDH活性显著增长,且各叶位对花前氮亏缺敏感度总体上表现为剑叶倒2叶倒3叶;破口期亏氮对籼型杂交稻中浙优1号上3叶氮同化酶活性的影响远小于减数分裂期处理,与之相比,籼粳杂交稻甬优12对破口期亏氮胁迫仍较敏感,表明中浙优1号植株氮代谢酶的亏氮敏感性由减数分裂期至破口期逐步下降,对土壤速效氮的需求同步降低,甬优12则对土壤供氮存在更高需求。     

8个格木天然林种源苗期生长差异与早期选择

为研究格木不同天然种源家系苗的生长差异,选出相对速生的优良种源,本研究对8个格木天然林种源、110个家系1年生的苗高和地径等生长量指标进行测量,结合各家系的种子表型性状进行方差分析、多重比较、相关性分析和聚类分析。结果表明:不同种源的格木苗期生长量差异显著,具备种源选择的基础;不同种源的苗期生长量差异主要来源于种源内,具备从优良种源内选择优良家系的基础;苗期生长量与种子大小、种子厚和千粒重等性状呈极显著正相关关系,大粒、饱满种子可作为优良种源和家系选择的初选参考指标。初步选出浦北、北流种源作为格木优良种源。本研究为格木速生种源及家系的早期选择提供依据。     

利用重组自交系高密度遗传图谱定位水稻芽期耐冷QTL

为定位水稻芽期耐冷QTL,本实验以双季超级稻品种‘五丰优T025’的双亲‘五丰B’和‘昌恢T025’杂交衍生的重组自交系(recombinant inbred lines, RILs)群体为材料,对10℃低温处理的水稻幼芽的存活率、根数、根长和芽长进行了测定。利用QTL Icimapping v4.2软件,共检测到3个控制芽期耐冷性QTL：qRL1、qRL2和qBL6,分别位于第1、2、6染色体上,LOD值分别为2.98,2.51和5.26,分别解释表型变异的10.54%,8.67%和14.04%,其增效等位基因均来自于亲本‘昌恢T025’。这些QTL定位在6.75k~40.05 kb染色体区间,为后续利用这些QTL进行分子标记辅助,选育芽期耐冷籼稻新品种奠定了基础。此外,检测到13对影响水稻芽期耐冷上位性互作QTL,分布在所有12条染色体,其中第3染色体与第8染色体之间互作位点可解释的表型变异率达到21.77%,表明上位性互作QTL在调控水稻芽期耐冷过程中也发挥了重要作用。     

气候变化对中国水稻生产的影响研究进展

水稻生产系统是响应气候变化最敏感的农业生态系统之一,本文综述了当前和未来气候变化对我国水稻生产的影响。气候变化背景下,我国水稻生长季的热量资源增多,辐射资源减少,降水不均一性加大。高温热害、干旱、暴雨和洪涝灾害发生更频繁,这可能降低水、热资源的有效性。气候变化使我国单季稻和双季稻潜在种植边界显著北移,导致单季稻、早稻和晚稻的主要生育期缩短。基于统计模型和水稻生长模型的研究结果表明,如果不考虑品种改良和栽培技术的进步,气候变化使单季稻、早稻和晚稻产量下降,但不同稻作区和方法间存在差异。我国水稻生产重心北移、实测生育期延长和产量增加的变化趋势,反映了水稻生产系统通过种植分布调整、品种改良和技术改进来适应气候变化的能力。未来气候变化将进一步导致水稻生育期缩短和产量下降,对我国水稻生产和粮食安全带来严峻挑战。仍需加强气候变化影响机制的研究及其在影响评估中的应用,减小影响评估的不确定性并增加其系统性,为制定有效的应对策略提供可靠的理论支持。     

山西南部苹果花期冻害时空分布特征及综合防御技术

利用1997—2016年山西南部苹果主产县(市、区)苹果花期逐日最低气温及日平均气温观测资料，根据苹果花期冻害等级划分指标，采用最小二乘法对花期冻害日数进行线性倾向估计，并用Mann-Kendall非参数检验方法对花期冻害日数的变化趋势进行显著性检验，分析山西南部苹果花期冻害的时空分布特征。结果表明: 山西省南部苹果产区发生花期冻害日数年均为3.1 d，各县花期均温介于13.0℃~13.8℃之间。运城地区4月中上旬易发生冻害，临汾地区吉县与隰县4月下旬易发生冻害；山西南部苹果花期冻害日数近20 a气候倾向率为-0.666 d·（10 a）^-1（P≤0.01），花期极端最低气温气候倾向率为0.165 d·（10 a）^-1（P≤0.01），花期极端最低气温与冻害日数具有极显著负相关关系。山西南部苹果花期冻害日数突变点在2008年，且在2015年之后苹果花期冻害日数突破α=0.05显著性水平下限。对于苹果花期冻害的综合防御措施，可采用“以防为主，抗补结合”策略。     

幼龄期压砂地枸杞产量和品质多指标耦合灌溉制度优化

为提高宁夏中部干旱带幼龄期压砂地枸杞灌水效率,保障枸杞产量和品质,进行了3水平灌水定额(300、225 m~3·hm~(-2)和150 m~3·hm~(-2))的田间对比试验,采用综合指标增量最优方法对灌溉制度进行了优化。研究发现:枸杞5月上旬萌芽期耗水以土壤蒸发为主,日耗水量0.6～1.2 mm,6月中旬进入夏果花期耗水量增大,日耗水量2.5～3.7 mm,7月中旬枸杞夏果期耗水量继续增大,达2.4～4.3 mm,7月下旬日耗水量最大,为3.2～4.7 mm,8月中旬耗水量下降,日耗水量3.0～4.2 mm。灌水定额对枸杞产量影响显著(P0.05),灌水定额300 m~3·hm~(-2)时产量最高,为1 402.65 kg·hm~(-2);灌水定额225 m~3·hm~(-2)时枸杞多糖、β-胡萝卜素和黄酮较高,含量分别为4.56%、0.15%和0.41%;灌水定额150 m~3·hm~(-2)时枸杞甜菜碱含量较高,为0.77%。进一步通过综合指标增量最优方法进行优化,结果表明β-胡萝卜素含量最高时得到综合指标增量最大,为-5.4%,总耗水量为450 mm。枸杞夏果花期进入需水关键期,阶段耗水量达70 mm以上,进入6月中旬枸杞需水量增大,旬耗水量达29 mm以上。可见,幼龄期压砂地枸杞产量及品质指标均达到最优的耗水量较难获得,但是在β-胡萝卜素含量最高时可以获得产量和品质多指标耦合的最优耗水量,据此进行灌溉制度优化,可以一定程度上实现幼龄期压砂地枸杞优质高产。     

苗后除草剂喷施时期对杂草防治及冬油菜产量和品质的影响

为了探究苗后除草剂不同施用时期对北方冬油菜田间杂草的防控效果及其对冬油菜生长、产量和品质的影响,以当地主栽甘蓝型冬油菜天油2266和白菜型冬油菜天油H614为试验材料,选用对冬油菜安全的苗后除草剂高效氟吡甲禾灵,设置4个喷施时期处理,于2018-2019年开展大田试验。结果表明,越冬后不同时期喷施苗后除草剂,甘蓝型冬油菜田间杂草总数较对照减少57.54%~92.56%,杂草总鲜重较对照降低75.40%~97.48%,第2时期（3月25日）处理产量较对照增加354.50kg/hm²;白菜型冬油菜田间杂草株防效和鲜重防效分别为55.91%~92.02%和37.17%~97.46%,产量较对照增加2.29%~16.77%。因此,在冬油菜大量返青但未封行前施用苗后除草剂,可显著防控田间杂草并增加冬油菜籽粒产量。     

紫穗槐幼苗不同生长阶段叶水势对干旱胁迫与复水的响应特征

为揭示紫穗槐（Amorpha fruticosa）幼苗叶水势对土壤干旱与复水环境的响应特征，本研究通过盆栽试验，研究了土壤干旱胁迫及复水对紫穗槐幼苗不同生长阶段叶水势的影响。结果表明：土壤水分、干旱历时和生长阶段3个因素以及各因素间的相互作用对紫穗槐幼苗叶水势均有极显著影响（P<0.01）；随着干旱程度加剧，幼苗叶水势呈下降趋势，水分胁迫指数呈上升趋势；40.00%土壤相对含水量下的幼苗叶水势下降幅度达到最大，其中干旱15 d的生长初期和生长中期以及干旱30 d的生长后期的叶水势分别比对照下降了0.71 MPa，1.12 MPa和0.58 MPa；复水后，叶水势出现补偿效应，随着复水后时间的延长，幼苗3个生长阶段叶水势逐渐恢复，恢复速率与干旱程度、干旱历时和生长阶段密切相关，但均在复水72小时后恢复到接近对照水平。